Sweet Sorghum Bagasse Bioethanol (Sweet Sorganol) Production by Simultaneous Saccharification and Fermentation by Trichoderma reesei and Saccharomyces cerevisiae

Essa Annisa Syadiah^1*, Khaswar Syamsu², Liesbetini Haditjaroko³

1. ¹ ² ³Bogor Agricultural University, Indonesia

Published in The Fourth NZAAR International Event Series on Natural and Built Environment, Cities, Sustainability and Advanced Engineering, Kuala Lumpur, Malaysia, 27 January 2018, pages 112 to 119. New Zealand Academy of Applied Research. ISSN 2463-5979 (online), 2463-5960 (print). Indexed in the Web of Science Core Collection, Conference Proceedings Citation Index (Clarivate).

Abstract

Bioethanol production from renewable resources has received great attention because of the increasing petroleum shortage. Demands of renewable energy from agricultural field residues increase because high population growth nowadays. Sweet sorghum bagasse (SSB) is one of the main agricultural residues in Egypt. So this study was performed on sweet sorghum bagasse as a resource for production of bioethanol. Pretreatment method for sweet sorghum bagasse by sodium hidroxyde (2%) hydrothermal and hydrogen peroxide (7,44%) in 6 hours with 120 rpm. Pretreated sweet sorgum bagasse was further enzymatically treated throughout simultaneous saccharification and fermentation (SSF). In previous studies, the hydrolisis and fermentation processed was performed separately using a separated hydrolisis ad fermentation (SHF) technique. This study processes via a SSF technique which produced higher substrate efficiency, cell yield , and product yield comapred to the SHF process. The charactericization process showed that pretreated SSB was contained higher cellulose than untreated cellulose. Sugar from the cellulose was produced by batch system cultivation for 84 hours using Trichoderma reesei. The highest sugar production was ahieved during the 36 hours. The production of bioethanol by SSF using Trichoderma reesei and Saccharomyces cerevisiae for 60 hours was 4,08 g/L of bioetanol, with a yield of bioethanol per substrate use (Yp/s) of 0,30 g ethanol/g substrate and a substrate conversion efficiency of 89,8%.

Keywords: Bioethanol; Trichodema reesei; Saccharomyces cerevisiae; Simultaneous sacharification and fermentation; sweet sorghum bagasse.5

Rights

Copyright © New Zealand Academy of Applied Research Ltd 2018. All rights reserved. Reproduced in the GDI Academy archive from the original proceedings without change to the authors’ text.